000			03354cam a22002414a 4500
008			1992
020			_a3540523286
040			_aGAMADERO _bSPA _cGAMADERO
041			_aeng
050	0	0	_aRA1250 _bP53 _c1992
100			_aH.F. Linskens _93322
245	0	0	_aPlant Toxin Analysis
250			_a1ra. edición
260	3		_aSpringer-Verlag _bAlemania _c1992
300			_a398pg. _bIlustrado _c16 x 24 cm
505			_aAnalytical Methods for Phytotoxins A. STIERLE, G. STROBEL, D. STIERLE, and F. SUGAWARA (With 4 Figures) 1 Introduction 2 Culturing Considerations 3 Bioassay Techniques 4 Separation Techniques 4.1 Column Chromatography 4.1.1 Gel Filtration Chromatography 4.1.2 Reverse Phase Chromatography 4.2 Centrifugal Countercurrent Chromatography. 5 Structure Elucidation Techniques 5.1 Nuclear Magnetic Resonance 5.2 Mass Spectrometry 5.2.1 Chemical Ionization 5.2.2 Fast Atom Bombardment References Quantitative Assays of Phytotoxins Using Plant Protoplasts and Isolated Cells E. GALUN and A. BREIMAN 1 Introduction ...... 2 Isolation and Culture of Cells and Protoplasts for Phytotoxin Bioassays 2.1 Establishment and Maintenance of Cultured Plant Cells for Phytotoxin Bioassays 2.2 Isolation and Culture of Protoplasts for Phytotoxin Bioassays 3 Specific Bioassays 3.1 Bioassay of Toxicity Imposed by a Culture-Filtrate from Xanthomonas campestris on Peach Mesophyll Cells ..... 3.1.1 General Considerations and Background 3.1.2 Protocol 3.2 The Evaluation of Toxicity of a Phytophthora citrophthora Culture Filtrate by Citrus Protoplasts 3.2.1 General Considerations and Background 3.2.2 Protocol ...... 3.3 Bioassay of Cercosporin by Tobacco Cells 3.3.1 General Considerations and Background 3.3.2 Protocol . 3.4 Bioassay of Sirodesmin by Brassica Protoplasts and Cells 3.4.1 General Considerations and Background 3.4.2 Protocol 3.5 Bioassay of Helminthosporium maydis Race T Toxin with Maize Protoplasts 3.5.1 General Considerations and Background 3.5.2 Protocol .. References Determination of Host-Selective Toxins К. Конмото 1 Introduction 2 Definition of Host-Selective Toxin 3 Bioassay of Host-Selective Toxins 3.1 Leaf Assay Electrolyte Leakage Assay CO2-Fixation Assay 3.9 Amino Acid Incorporation Assay 3.10 Infection Induction Assay .. 4 HPLC Analysis of Host-Selective Toxins 4.1 AK-Toxin and AF-Toxin .... 4.2 ACT-Toxin
520			_aChemical defence by means of toxins poisonous to other organisms, be they animals or plants, is widespread amongst the plant kingdom - including microorganisms as well. This book embraces the analysis of a wide range of plant toxins and this fills a gap in the plant pathology and ecological biochemistry fields. The topics covered include toxic extracellular enzymes, host selective toxins, elicitors, phototoxins, aflatoxins, mycotoxins, and ecotoxic substance tests by pollen germination and growth. The analytical procedures, which are used to evaluate the toxins, are covered in such a way that the reader is able to carry them out mostly solely by following the detailed descriptions.
526			_aIngeniería Ambiental
650		0	_aBiología _995
700			_aJ.F. Jackson _93936
942			_2lcc _cLIB _e1ra. edición _n0
945			_a1260 _bNorma Gabriela Corona Arreguin _c1260 _dNorma Gabriela Corona Arreguin
999			_c8499 _d8499