Plant Toxin Analysis

Analytical Methods for Phytotoxins
A. STIERLE, G. STROBEL, D. STIERLE, and F. SUGAWARA (With 4 Figures)
1 Introduction
2 Culturing Considerations
3 Bioassay Techniques
4 Separation Techniques
4.1 Column Chromatography
4.1.1 Gel Filtration Chromatography
4.1.2 Reverse Phase Chromatography
4.2 Centrifugal Countercurrent Chromatography.
5 Structure Elucidation Techniques
5.1 Nuclear Magnetic Resonance
5.2 Mass Spectrometry
5.2.1 Chemical Ionization
5.2.2 Fast Atom Bombardment
References
Quantitative Assays of Phytotoxins Using Plant Protoplasts and Isolated Cells
E. GALUN and A. BREIMAN
1 Introduction ......
2 Isolation and Culture of Cells and Protoplasts for Phytotoxin Bioassays
2.1 Establishment and Maintenance of Cultured Plant Cells for Phytotoxin Bioassays
2.2 Isolation and Culture of Protoplasts for Phytotoxin Bioassays
3 Specific Bioassays
3.1 Bioassay of Toxicity Imposed by a Culture-Filtrate from Xanthomonas campestris on Peach Mesophyll Cells .....
3.1.1 General Considerations and Background
3.1.2 Protocol
3.2
The Evaluation of Toxicity of a Phytophthora citrophthora
Culture Filtrate by Citrus Protoplasts
3.2.1 General Considerations and Background
3.2.2 Protocol ......
3.3
Bioassay of Cercosporin by Tobacco Cells
3.3.1 General Considerations and Background
3.3.2 Protocol .
3.4
Bioassay of Sirodesmin by Brassica Protoplasts and Cells
3.4.1 General Considerations and Background
3.4.2 Protocol
3.5
Bioassay of Helminthosporium maydis Race T Toxin with Maize Protoplasts
3.5.1 General Considerations and Background
3.5.2 Protocol ..
References
Determination of Host-Selective Toxins
К. Конмото
1 Introduction
2 Definition of Host-Selective Toxin
3 Bioassay of Host-Selective Toxins
3.1 Leaf Assay
Electrolyte Leakage Assay
CO2-Fixation Assay
3.9 Amino Acid Incorporation Assay
3.10 Infection Induction Assay ..
4 HPLC Analysis of Host-Selective Toxins
4.1 AK-Toxin and AF-Toxin ....
4.2 ACT-Toxin

Chemical defence by means of toxins poisonous to other
organisms, be they animals or plants, is widespread amongst
the plant kingdom - including microorganisms as well. This
book embraces the analysis of a wide range of plant toxins
and this fills a gap in the plant pathology and ecological
biochemistry fields. The topics covered include toxic
extracellular enzymes, host selective toxins, elicitors,
phototoxins, aflatoxins, mycotoxins, and ecotoxic substance
tests by pollen germination and growth. The analytical
procedures, which are used to evaluate the toxins, are
covered in such a way that the reader is able to carry them
out mostly solely by following the detailed descriptions.

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