H.F. Linskens  <br/><br/>
Plant Toxin Analysis 

 - 1ra. edición  
 - Springer-Verlag  Alemania   1992 
 - 398pg.  Ilustrado  16 x 24 cm  
<br/><br/>Analytical Methods for Phytotoxins<br/>A. STIERLE, G. STROBEL, D. STIERLE, and F. SUGAWARA (With 4 Figures)<br/>1 Introduction<br/>2 Culturing Considerations<br/>3 Bioassay Techniques<br/>4 Separation Techniques<br/>4.1 Column Chromatography<br/>4.1.1 Gel Filtration Chromatography<br/>4.1.2 Reverse Phase Chromatography<br/>4.2 Centrifugal Countercurrent Chromatography.<br/>5 Structure Elucidation Techniques<br/>5.1 Nuclear Magnetic Resonance<br/>5.2 Mass Spectrometry<br/>5.2.1 Chemical Ionization<br/>5.2.2 Fast Atom Bombardment<br/>References<br/>Quantitative Assays of Phytotoxins Using Plant Protoplasts and Isolated Cells<br/>E. GALUN and A. BREIMAN<br/>1 Introduction ......<br/>2 Isolation and Culture of Cells and Protoplasts for Phytotoxin Bioassays<br/>2.1 Establishment and Maintenance of Cultured Plant Cells for Phytotoxin Bioassays<br/>2.2 Isolation and Culture of Protoplasts for Phytotoxin Bioassays<br/>3 Specific Bioassays<br/>3.1 Bioassay of Toxicity Imposed by a Culture-Filtrate from Xanthomonas campestris on Peach Mesophyll Cells .....<br/>3.1.1 General Considerations and Background<br/>3.1.2 Protocol<br/>3.2<br/>The Evaluation of Toxicity of a Phytophthora citrophthora<br/>Culture Filtrate by Citrus Protoplasts<br/>3.2.1 General Considerations and Background<br/>3.2.2 Protocol ......<br/>3.3<br/>Bioassay of Cercosporin by Tobacco Cells<br/>3.3.1 General Considerations and Background<br/>3.3.2 Protocol .<br/>3.4<br/>Bioassay of Sirodesmin by Brassica Protoplasts and Cells<br/>3.4.1 General Considerations and Background<br/>3.4.2 Protocol<br/>3.5<br/>Bioassay of Helminthosporium maydis Race T Toxin with Maize Protoplasts<br/>3.5.1 General Considerations and Background<br/>3.5.2 Protocol ..<br/>References<br/>Determination of Host-Selective Toxins<br/>К. Конмото<br/>1 Introduction<br/>2 Definition of Host-Selective Toxin<br/>3 Bioassay of Host-Selective Toxins<br/>3.1 Leaf Assay<br/>Electrolyte Leakage Assay<br/>CO2-Fixation Assay<br/>3.9 Amino Acid Incorporation Assay<br/>3.10 Infection Induction Assay ..<br/>4 HPLC Analysis of Host-Selective Toxins<br/>4.1 AK-Toxin and AF-Toxin ....<br/>4.2 ACT-Toxin 
<br/><br/> Chemical defence by means of toxins poisonous to other<br/>organisms, be they animals or plants, is widespread amongst<br/>the plant kingdom - including microorganisms as well. This<br/>book embraces the analysis of a wide range of plant toxins<br/>and this fills a gap in the plant pathology and ecological<br/>biochemistry fields. The topics covered include toxic<br/>extracellular enzymes, host selective toxins, elicitors,<br/>phototoxins, aflatoxins, mycotoxins, and ecotoxic substance<br/>tests by pollen germination and growth. The analytical<br/>procedures, which are used to evaluate the toxins, are<br/>covered in such a way that the reader is able to carry them<br/>out mostly solely by following the detailed descriptions. 
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<br/><br/><label>ISBN: </label>3540523286 
<br/><br/><label>Subjects--Topical Terms: </label><br/>Biología
<br/><br/><label>LC Class. No.: </label>RA1250  / P53